Caveolin 2 Phospho (pY19) (CAV2)_珈得尔化学试剂

【产品介绍】Caveolins (Caveolin 1, 2, and 3) are a family
of caveolae-associated integral membrane proteins.
Caveolae are cholesterol/sphingolipid-rich microdomains
of the plasma membrane that have been implicated in
signal transduction and vesicular trafficking (1).
Caveolin 2 is a principal component of caveolae
membranes. It interacts directly with heterotrimeric
guanine nucleotide binding proteins (G proteins) and
can functionally regulate their activity. Caveolins 1
and 2 are similar in most respects; however, they differ
in their functional interactions with heterotrimeric
G proteins, possibly explaining why Caveolin-1 and
-2 are coexpressed within a single cell (2).
Caveolin-2 is up-regulated in response to the
mechanical injury of differentiated PC12 cells, which
is strictly dependent on continued treatment with NGF.
Both Caveolin-1 and -2 are more abundantly expressed
in white adipose tissue and are induced during
adipocyte differentiation. Robust expression of
Caveolin-1 and -2 is also observed along the entire
cell surface of DRG neurons, including high levels on
growth cones (1). Phosphorylation on Tyr-19 is required
for insulin-induced phosphorylation of MAPK1 and DNA
binding of STAT3 (2).

Recommended Applications

WB, FC
Applications and Recommended Dilution Factors

WB: 1:1,000 - 10,000
FC: 1:10 - 100
Species Reactivity *

Human

*Species cross-reactivity is based on WB analysis.

Products Data

Western blot analysis on HUVEC cell lysates
using anti-Phospho-Caveolin 2 (pY19) RabMAb
(cat. #3873-1). Cells were either (A) untreated (B)
treated with pervanadate.

Flow cytometric analysis of permeabilized HUVEC
cells, untreated (green) or pervanadate-treated (red)
using anti-Caveolin 2 Phospho (pY19) RabMAb
(catalog # 3873-1), and pervanadate-treated HUVEC
cells using the same antibody preincubated with
phospho-Caveolin 2 Phospho (pY19) peptide (blue)
or non-phospho-Caveolin 2 Phospho (pY19) peptide
(orange).

Specificity

A phospho specific peptide corresponding to
residues surrounding tyrosine 19 of human Caveolin 2.
This antibody detects Caveloin 2 phosphorylated at
tyrosine 19.

贮存：

Storage Condition and Buffer

Store at -20° C. Buffer: 50 mM Tris-Glycine (pH 7.4),
0.15 M NaCl, 40% Glycerol, 0.01% sodium azide and
0.05% BSA. Stable for 12 months from date of receipt.