Recombinant Human Stem Cell Factor (P.pastoris-expressed)，是 c-kit 配体，是一种含二硫键的细胞因子，用于调节造血细胞和其他细胞如肥大细胞，生殖细胞的发育和功能。
Synonyms
rHuSCF; Hematopoietic growth factor KL; MGF; Mast Cell Growth Factor
Species
HumanSource
P. pastoris Accession
P21583 Gene ID
4254 Molecular Weight
Approximately 20 kDa AA Sequence
MEGICRNRVT NNVKDVTKLV ANLPKDYMIT LKYVPGMDVL PSHCWISEMV VQLSDSLTDL LDKFSNISEG LSNYSIIDKL VNIVDDLVEC VKENSSKDLK KSFKSPEPRL FTPEEFFRIF NRSIDAFKDF VVASETSDCV VSSTLSPEKD SRVSVTKPFM LPPVA Biological Activity
The ED50 is <2 ng/mL as measured by human TF-1 cells, corresponding to a specific activity of 5 × 105 units/mg. Appearance
Lyophilized powder. Formulation
Lyophilized after extensive dialysis against 10 mM acetic acid. Endotoxin Level
<1 EU/μg, determined by LAL method. Reconstitution
Reconstitute the lyophilized recombinant Human Stem Cell Factor (P.pastoris-expressed) (rHuSCF) to 100 µg/mL using ddH2O. Storage & Stability
Lyophilized recombinant Human Stem Cell Factor (P.pastoris-expressed) (rHuSCF) is stored at -20°C. After reconstitution, it is stable at 4°C for 4 weeks or -20°C for longer. It is recommended to freeze aliquots at -20°C or -80°C for extended storage. Shipping
Room temperature in continental US; may vary elsewhere. Background
The recombinant human stem cell factor (rhSCF) protein specifically enhances the viability of human myeloid leukemia cell line TF-1 and the proliferation and maturation of human mast cell line LAD2 cell. Recombinant human SCF (rhSCF) can maintain hematopoietic stem cells and mast cell in culture, and it is widely tested in research into differentiation from embryonic stem cell (ESC)/induced pluripotent stem cell (iPSC)[1]. |
