Recombinant Rat Stem Cell Factor (P.pastoris-expressed) 是一种造血因子，与它的配体 c-kit 结合，在造血、精子发生和黑素生成中起着重要的作用。
Synonyms
rRtSCF; Hematopoietic growth factor KL; MGF; Mast Cell Growth Factor ; 重组大鼠干细胞因子 (P.pastoris 表达)
Species
RatSource
P. pastoris Accession
P21581 Gene ID
60427 Molecular Weight
Approximately 18.3 kDa AA Sequence
QEICRNPVTD NVKDITKLVA NLPNDYMITL NYVAGMDVLP SHCWLRDMVT HLSVSLTTLL DKFSNISEGL SNYSIIDKLG KIVDDLVACM EENAPKNVKE SLKKPETRNF TPEEFFSIFN RSIDAFKDFM VASDTSDCVL SSTLGPEKDS RVSVTKPFML PPVA Biological Activity
The ED50 is <25 ng/mL as measured by TF-1 cells, corresponding to a specific activity of >4 × 104 units/mg. Appearance
Lyophilized powder. Formulation
Lyophilized after extensive dialysis against PBS. Endotoxin Level
<0.2 EU/μg, determined by LAL method. Reconstitution
Reconstitute the lyophilized recombinant Rat Stem Cell Factor (P.pastoris-expressed) (rRtSCF) to 100 µg/mL using ddH2O. Storage & Stability
Lyophilized recombinant Rat Stem Cell Factor (P.pastoris-expressed) (rRtSCF) is stored at -20°C. After reconstitution, it is stable at 4°C for 2 weeks or -20°C for longer. It is recommended to freeze aliquots at -20°C or -80°C for extended storage. Shipping
Room temperature in continental US; may vary elsewhere. Background
Stem Cell Factor is a hematopoietic cytokine, triggered by binding to its ligand c-kit, and plays an important role in hematopoiesis, spermatogenesis, and melanogenesis. Stem Cell Factor (SCF) is applied for hematopoietic stem cell mobilization, exvivo stem/progenitor cell expansion, gene therapy, and immunotherapy[1]. Stem Cell Factor regulates cell survival, proliferation, differentiation, and migration in divergent cell types[2]. |
