重组人表皮生长因子 (Recombinant Human Epidermal Growth Factor) 是一种重要的表皮生长因子，能够刺激表皮细胞增殖，可应用于伤口愈合的研究。
Synonyms
rHuEGF; Pro-epidermal growth factor; Urogastrone ; 重组人表皮生长因子
Species
HumanSource
E. coli Accession
P01133 Gene ID
1950 Molecular Weight
Approximately 6.2 kDa AA Sequence
MNSDSECPLS HDGYCLHDGV CMYIEALDKY ACNCVVGYIG ERCQYRDLKW WELR Biological Activity
The ED50 is <0.2 ng/mL as measured by murine BALB/c 3T3 cells, corresponding to a specific activity of 5.0 × 106 units/mg. Appearance
Lyophilized powder. Formulation
Lyophilized after extensive dialysis against 10mM Phosphate buffer, pH 7.0, 200 mM NaCl buffer. Endotoxin Level
<0.2 EU/μg, determined by LAL method. Reconstitution
Reconstitute the lyophilized recombinant Human Epidermal Growth Factor (rHuEGF) to 100 µg/mL using ddH2O. Storage & Stability
Lyophilized recombinant Human Epidermal Growth Factor (rHuEGF) is stored at -20°C. After reconstitution, it is stable at 4°C for 4 weeks or -20°C for longer. It is recommended to freeze aliquots at -20°C or -80°C for extended storage. Shipping
Room temperature in continental US; may vary elsewhere. Background
Recombinant Human Epidermal Growth Factor is chemically identical to the natural material, exhibits full biological activity and is ued in wound healing applications[1]. Recombinant Human Epidermal Growth Factor (rhEGF) stimulates proliferation of the fibroblast BALB/c3T3 cell line. Recombinant Human Epidermal Growth Factor released from hydrogels keeps its bioactivity, induces EGF receptor expression, causes proliferating cell nuclear antigen and shows therapeutic potential in enhancing diabetic wound healing[2]. |
