Recombinant Mouse Sonic Hedgehog, C25II 是一种小鼠重组 sonic hedgehog 的变体。
Synonyms
rMuSonic Hedgehog, C25II; Hhg1 ; 重组小鼠 Sonic Hedgehog, C25II
Species
MouseSource
E. coli Accession
Q62226 Gene ID
20423 Molecular Weight
Approximately 19.7 kDa AA Sequence
IIGPGRGFGK RRHPKKLTPL AYKQFIPNVA EKTLGASGRY EGKITRNSER FKELTPNYNP DIIFKDEENT GADRLMTQRC KDKLNALAIS VMNQWPGVKL RVTEGWDEDG HHSEESLHYE GRAVDITTSD RDRSKYGMLA RLAVEAGFDW VYYESKAHIH CSVKAENSVA AKSGG Biological Activity
The ED50 is <2 μg/mL as measured by C3H/10T1/2 (CCL-226) cells, corresponding to a specific activity of >500 units/mg. Appearance
Lyophilized powder. Formulation
Lyophilized after extensive dialysis against PBS. Endotoxin Level
<0.2 EU/μg, determined by LAL method. Reconstitution
Reconstitute the lyophilized recombinant Mouse Sonic Hedgehog, C25II (rMuSonic Hedgehog, C25II) to 100 µg/mL using ddH2O. Storage & Stability
Lyophilized recombinant Mouse Sonic Hedgehog, C25II (rMuSonic Hedgehog, C25II) is stored at -20°C. After reconstitution, it is stable at 4°C for 2 weeks or -20°C for longer. It is recommended to freeze aliquots at -20°C or -80°C for extended storage. Shipping
Room temperature in continental US; may vary elsewhere. Background
Sonic Hedgehog (SHH) is a secreted factor. The protein is heavily modified through cleavage and posttranslational modification and its release from producing cells can also be modulated by interactions with Dispatched, Scube2, and heparan sulfate proteoglycan. Sonic hedgehog (SHH) is the main ligand of hedgehog (Hh) pathway in mouse[1][2]. |
